Molecular Detection Division
One of the methods of DNA replication is the use of the PCR method. Due to its many uses and advantages, it has rapidly expanded in molecular laboratories and is now commonly used in these laboratories.
In 1983, Mollis worked on a polymerase chain reaction to amplify certain DNA sequences. In terms of practical principles, PCR has a great similarity to DNA replication and is actually taken from it. The PCR technique consists of repetitive cycles in which the DNA is heated and cooled with the help of primers, the heat-resistant DNA polymerase enzyme is suitable for buffering and iM + 2 Mg and in the presence of four dNTPs (DNA) constructors acting on DNA template Performs replication. The DNA replication process is exponential in this way, and thus a large amount of DNA can be obtained. Primers complement parts of the two strands of DNA and determine the region to be multiplied.
Today, the PCR method has a very important role in identifying and isolating genes, cloning, molecular biology,
Diagnostic microbiology, diagnosis of cancer and genetic diseases, identification, criminology, sequencing, archeology, evolutionary study of organisms.