Electrophoresis, is the movement of solute-containing molecules by opposite the passage of an electric field of the desired composition to the opposite direction to the electrode. Because of the difference in electrical charge, shape and size, molecules and components of a compound migrate to different speeds, so they are separated into separate parts, hence electrophoresis is used as a simple and quick analysis tool. Takes. This method is used not only for the analysis and purification of very large molecules, such as proteins and nucleic acids, but also for easier pregnant molecules, including pregnant sugars, amino acids, peptides, nucleotides, and simple ions. Electrophoresis is used in biological and biochemical research, chemistry of proteins, pharmacology, forensic medicine, clinical findings, veterinary science, food control, as well as molecular biology.
Serum protein electrophoresis is an easy and inexpensive way to isolate and quantitatively test serum and urine proteins based on their pure weight, size and shape. My serum contains more than 1000 proteins, each of which has its own special functions and their concentration varies under different pathological conditions. Serum proteins are divided into five distinct parts, including albumin, alpha-1 globulin (α1), α2-globulin (α2), β-beta- and gamma globulin (γ) based on their charge.
Evaluation of serum protein components to detect, investigate and control the course of cancer patients (lymphoma, myeloma), diseases
Intestine-kidney with protein excretion, liver disorders, immune deficiencies, monoclonal gammapathies, multiple myeloma, macroglobulinemia, valvular stroma, malnutrition and chronic diseases associated with edema.
The process of isolating proteins involves placing them in a matrix and then observing the motion of the protein in the presence of an electric field. Proteins are composed of covalent amino acids and have different sizes and electrical loads, so they can be isolated electrochemically. Proteins are negatively charged in an alkaline buffer (pH = 5.8-9) and migrate from the negative electrode to the positive electrode. The electrophoresis matrix is an agarose gel and proteins are separated from each other based on their superficial load density.